| Birmingham Legion FC vs. Louisville City FC | 3-15-2025
Saturday
7:00 PM | Protective Stadium in Birmingham, AL | View Tickets |
| Louisville City FC vs. Loudoun United FC | 3-22-2025
Saturday
4:00 PM | Lynn Family Stadium in Louisville, KY | View Tickets |
| Louisville City FC vs. Detroit City FC | 3-29-2025
Saturday
4:00 PM | Lynn Family Stadium in Louisville, KY | View Tickets |
| Sacramento Republic FC vs. Louisville City FC | 4-5-2025
Saturday
7:00 PM | Heart Health Park in Sacramento, CA | View Tickets |
| Tampa Bay Rowdies vs. Louisville City FC | 4-19-2025
Saturday
7:30 PM | Al Lang Stadium in St. Petersburg, FL | View Tickets |
| Louisville City FC vs. Richmond Kickers SC | 4-26-2025
Saturday
7:30 PM | Lynn Family Stadium in Louisville, KY | View Tickets |
| Louisville City FC vs. Lexington Sporting Club | 5-1-2025
Thursday
8:00 PM | Lynn Family Stadium in Louisville, KY | View Tickets |
| Louisville City FC vs. Pittsburgh Riverhounds SC | 5-10-2025
Saturday
7:30 PM | Lynn Family Stadium in Louisville, KY | View Tickets |
| Hartford Athletic FC vs. Louisville City FC | 5-17-2025
Saturday
7:00 PM | Trinity Health Stadium in Hartford, CT | View Tickets |
| Monterey Bay FC vs. Louisville City FC | 5-24-2025
Saturday
7:00 PM | Cardinale Stadium in Marina, CA | View Tickets |
| Angel City FC vs. Racing Louisville FC | 5-24-2025
Saturday
7:00 PM | BMO Stadium in Los Angeles, CA | View Tickets |
| Loudoun United FC vs. Louisville City FC | 5-31-2025
Saturday
6:00 PM | Segra Field in Leesburg, VA | View Tickets |
| Louisville City FC vs. Tampa Bay Rowdies | 6-7-2025
Saturday
8:00 PM | Lynn Family Stadium in Louisville, KY | View Tickets |
| Louisville City FC vs. Rhode Island FC | 6-11-2025
Wednesday
8:00 PM | Lynn Family Stadium in Louisville, KY | View Tickets |
| Kansas City Current vs. Racing Louisville FC | 6-14-2025
Saturday
6:30 PM | CPKC Stadium in Kansas City, MO | View Tickets |
| Louisville City FC vs. Birmingham Legion FC | 6-14-2025
Saturday
8:00 PM | Lynn Family Stadium in Louisville, KY | View Tickets |
| Loudoun United FC vs. Louisville City FC | 6-25-2025
Wednesday
6:00 PM | Segra Field in Leesburg, VA | View Tickets |
| Louisville City FC vs. North Carolina FC | 6-28-2025
Saturday
8:00 PM | Lynn Family Stadium in Louisville, KY | View Tickets |
| Colorado Springs Switchbacks FC vs. Louisville City FC | 7-4-2025
Friday
6:00 PM | Weidner Field in Colorado Springs, CO | View Tickets |
| Louisville City FC vs. FC Tulsa | 7-19-2025
Saturday
8:00 PM | Lynn Family Stadium in Louisville, KY | View Tickets |
| Lexington Sporting Club vs. Louisville City FC | 7-26-2025
Saturday
7:00 PM | Lexington SC Stadium in Lexington, KY | View Tickets |
| Racing Louisville FC vs. Kansas City Current | 8-1-2025
Friday
8:00 PM | Lynn Family Stadium in Louisville, KY | View Tickets |
| Louisville City FC vs. North Carolina FC | 8-2-2025
Saturday
8:00 PM | Lynn Family Stadium in Louisville, KY | View Tickets |
| Orlando Pride vs. Racing Louisville FC | 8-9-2025
Saturday
7:30 PM | Inter&Co Stadium in Orlando, FL | View Tickets |
| Louisville City FC vs. Charleston Battery | 8-9-2025
Saturday
8:00 PM | Lynn Family Stadium in Louisville, KY | View Tickets |
| Miami FC vs. Louisville City FC | 8-16-2025
Saturday
6:40 PM | Pitbull Stadium in Miami, FL | View Tickets |
| Louisville City FC vs. New Mexico United | 8-23-2025
Saturday
8:00 PM | Lynn Family Stadium in Louisville, KY | View Tickets |
| Indy Eleven vs. Louisville City FC | 8-30-2025
Saturday
7:00 PM | Carroll Stadium in Indianapolis, IN | View Tickets |
| Rhode Island FC vs. Louisville City FC | 9-6-2025
Saturday
4:00 PM | Tidewater Landing Stadium in Pawtucket, RI | View Tickets |
| Pittsburgh Riverhounds SC vs. Louisville City FC | 9-13-2025
Saturday
7:00 PM | Highmark Stadium in Pittsburgh, PA | View Tickets |
| Utah Royals vs. Racing Louisville FC | 9-19-2025
Friday
8:00 PM | America First Field in Sandy, UT | View Tickets |
| Louisville City FC vs. Las Vegas Lights FC | 9-20-2025
Saturday
7:30 PM | Lynn Family Stadium in Louisville, KY | View Tickets |
| Detroit City FC vs. Louisville City FC | 9-27-2025
Saturday
7:00 PM | Keyworth Stadium in Hamtramck, MI | View Tickets |
| Racing Louisville FC vs. Angel City FC | 9-27-2025
Saturday
7:30 PM | Lynn Family Stadium in Louisville, KY | View Tickets |
| Louisville City FC vs. Indy Eleven | 10-4-2025
Saturday
7:30 PM | Lynn Family Stadium in Louisville, KY | View Tickets |
| Louisville City FC vs. Miami FC | 10-11-2025
Saturday
7:30 PM | Lynn Family Stadium in Louisville, KY | View Tickets |
| Orange County SC vs. Louisville City FC | 10-18-2025
Saturday
5:00 PM | Championship Soccer Stadium at OC Great Park in Irvine, CA | View Tickets |
| NJ/NY Gotham FC vs. Racing Louisville FC | 10-19-2025
Sunday
3:00 PM | Sports Illustrated Stadium in Harrison, NJ | View Tickets |
| Louisville City FC vs. Hartford Athletic FC | 10-25-2025
Saturday
7:30 PM | Lynn Family Stadium in Louisville, KY | View Tickets |
